The Identification of Novel Cell Death Inducers by Jacob Baus

Dublin Core

Title

The Identification of Novel Cell Death Inducers by Jacob Baus

Subject

Biology

Description

Cell death caused by cardiac infarction events commonly result in the development of heart disease. The identification and classification of genes in these cell death pathways improves medical professionals understanding of these mechanisms and helps in the development of gene therapy techniques which can be used to impede the onset of heart disease. The goal of this research was to identify novel genes in cell death pathways. We amplified cDNA for 10 genes which were screened for high death potential. This amplified cDNA was then added into an inducible vector and inserted into an adenovirus. This adenovirus was then introduced to cells with the doxycycline (DOX) inducer and examined for cell death. We expected to see cells that had virus applied without DOX to be healthy and living, and cells with virus and DOX to have significant cell death from the induced gene. Instead, we observed substantial cell death in both cases of virus applied to cells and healthy, living control cells. We concluded that the induced gene could not be responsible for this death and rather viral toxicity caused the increased death. Further testing these viruses, with consideration of recent literature, could demonstrate if these genes are active in a cell death pathway.

Creator

Jacob Baus

Source

Senior Showcase Poster presentation

Publisher

Ripon College

Date

April 17, 2018

Rights

The author reserves all rights.

Format

pdf

Identifier

Major: Biology
Minor: Chemistry
Mount Calvary, Wisconsin
BIO 502: Biology Senior Seminar

Files

Identification of Novel Cell Death Inducers (Final Draft).pdf
Baus - Senior Seminar Poster.pdf

Citation

Jacob Baus, “The Identification of Novel Cell Death Inducers by Jacob Baus,” Senior Showcase Digital Collection, accessed May 20, 2018, http://rcseniorshowcase.omeka.net/items/show/82.

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