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Dublin Core
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Title
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Senior Showcase 2019
Description
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Oral and poster presentations from Senior Showcase held on April 23, 2019 at Ripon College.
Publisher
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Ripon College Lane Library
Date
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April 23, 2019
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Ripon College Seniors 2019
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Dublin Core
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Title
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Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez
Subject
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Biology
Description
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Transcription termination is an important regulatory component within the Escherichia coli (E. coli) genome. Two proteins are known to influence transcriptional termination in E. coli: Rho and NusA. Rho only functions to induce transcription termination, while NusA is involved in both the enhancement and inhibition of termination. This study aimed to determine whether the two proteins influence each other's functions through direct or indirect interactions. Reciprocal yeast two hybrid (Y2H) analysis using vectors expressing Rho and NusA as fusion with the protein domain of the Gal4 transcriptional factor indicated direct interactions between the two proteins. These results will allow us to further study the mechanistic roles of each protein in transcription termination, as well as the process of gene expression throughout the E.coli genome.
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Bosquez, Taryn
Source
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Senior Showcase Oral presentation
Publisher
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Ripon College
Date
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April 23, 2019
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pdf
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Major: Biology
Minor: Psychology
Omro, Wisconsin
BIO 502
Biology
pausing
Rho-dependent termination
RNA polymerase
yeast two hybrid system