Description
Transcription termination is an important regulatory component within the Escherichia coli (E. coli) genome. Two proteins are known to influence transcriptional termination in E. coli: Rho and NusA. Rho only functions to induce transcription termination, while NusA is involved in both the enhancement and inhibition of termination. This study aimed to determine whether the two proteins influence each other's functions through direct or indirect interactions. Reciprocal yeast two hybrid (Y2H) analysis using vectors expressing Rho and NusA as fusion with the protein domain of the Gal4 transcriptional factor indicated direct interactions between the two proteins. These results will allow us to further study the mechanistic roles of each protein in transcription termination, as well as the process of gene expression throughout the E.coli genome.