Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez

Dublin Core

Title

Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez

Subject

Biology

Description

Transcription termination is an important regulatory component within the Escherichia coli (E. coli) genome. Two proteins are known to influence transcriptional termination in E. coli: Rho and NusA. Rho only functions to induce transcription termination, while NusA is involved in both the enhancement and inhibition of termination. This study aimed to determine whether the two proteins influence each other's functions through direct or indirect interactions. Reciprocal yeast two hybrid (Y2H) analysis using vectors expressing Rho and NusA as fusion with the protein domain of the Gal4 transcriptional factor indicated direct interactions between the two proteins. These results will allow us to further study the mechanistic roles of each protein in transcription termination, as well as the process of gene expression throughout the E.coli genome.

Creator

Bosquez, Taryn

Source

Senior Showcase Oral presentation

Publisher

Ripon College

Date

April 23, 2019

Rights

The author reserves all rights.

Format

pdf

Identifier

Major: Biology
Minor: Psychology
Omro, Wisconsin
BIO 502

Files

Senior Sem Thesis-10.pdf

Citation

Bosquez, Taryn, “Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez,” Senior Showcase Digital Collection, accessed December 6, 2022, https://rcseniorshowcase.omeka.net/items/show/114.

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