Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez
Dublin Core
Title
Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez
Subject
Biology
Description
Transcription termination is an important regulatory component within the Escherichia coli (E. coli) genome. Two proteins are known to influence transcriptional termination in E. coli: Rho and NusA. Rho only functions to induce transcription termination, while NusA is involved in both the enhancement and inhibition of termination. This study aimed to determine whether the two proteins influence each other's functions through direct or indirect interactions. Reciprocal yeast two hybrid (Y2H) analysis using vectors expressing Rho and NusA as fusion with the protein domain of the Gal4 transcriptional factor indicated direct interactions between the two proteins. These results will allow us to further study the mechanistic roles of each protein in transcription termination, as well as the process of gene expression throughout the E.coli genome.
Creator
Bosquez, Taryn
Source
Senior Showcase Oral presentation
Publisher
Ripon College
Date
April 23, 2019
Rights
The author reserves all rights.
Format
pdf
Identifier
Major: Biology
Minor: Psychology
Minor: Psychology
Omro, Wisconsin
BIO 502
Files
Collection
Citation
Bosquez, Taryn, “Direct interactions between Escherichia coli transcription proteins Rho and NusA by Taryn Bosquez,” Senior Showcase Digital Collection, accessed April 19, 2024, https://rcseniorshowcase.omeka.net/items/show/114.